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Interactions of polyomavirus structures with components of cell innate immunity
Portychová, Tereza ; Forstová, Jitka (advisor) ; Schreiberová, Lucie (referee)
The topic of this thesis are the interactions of polyomavirus structures with components of innate immunity in infected cells. This review is focused on model SV40 and MPyV polyomaviruses and human BKPyV, JCPyV and MCPyV. The research of the interplay of innate immunity response and polyomaviruses is in its infancy. Infection with all studied polyomaviruses induces, via their LT antigens, DNA damage response (DDR), necessary for their efficient replication. DDR can activate both the canonical and the non-canonical pathway of interferon induction leading to an antiviral state. Polyomaviruses are recognized by the immune system first during replication of their genomes. Interferon induction by polyomaviruses can be initiated by the DNA sensor, cGAS, followed by STING activation, but also by recognition of the viral RNA by the RIG-1 sensor. The virus early LT and st antigens and the late agnoprotein of some polyomaviruses have demonstrated the potential to regulate innate immune responses and thus contribute to the establishment of polyomavirus persistence. Keywords: polyomaviruses, innate cell immunity, large T antigen, small t antigen, agnoprotein, interferon-stimulated genes
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Properties and functions of agnoprotein of polyomaviruses
Zosinčuková, Tereza ; Forstová, Jitka (advisor) ; Vinšová, Barbora (referee)
Polyomaviridae family includes small DNA viruses with simple structure and a small genome encoding only a few proteins. These proteins include large T and small T antigens, as well as 2 to 3 structural proteins known as VP1, VP2 and VP3. In addition, some members of the Polyomaviridae family encode in their genome a small non-structural protein called agnoprotein. Among human polyomaviruses, agnoprotein is present in BK polyomavirus and JC polyomavirus. These viruses are the causative agents of some serious diseases in immunocompromised humans and therefore, they are the subject of intensive research. Simian vacuolating virus 40 is another example of a virus which encodes the agnoprotein. Agnoprotein is capable to manipulate its host cell, disrupt vesicle transport and is also crucial for viral replication and transcription. It appears to play an important role in the morphogenesis of virions and/or in their release from the cell. This paper comprehensively summarizes the latest insights into the properties and functions of the agnoprotein BK polyomavirus, JC polyomavirus and SV40 virus, focusing on the production of this protein during infection, its structure, posttranslational modifications, cell localization, interaction partners and the overall importance of this enigmatic protein for the...
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